Journal article
Matching sensitivity to abundance: High resolution immuno-mass spectrometry imaging of lanthanide labels and endogenous elements in the murine brain
D Clases, R Gonzalez De Vega, S Funke, TE Lockwood, MT Westerhausen, RV Taudte, PA Adlard, PA Doble
Journal of Analytical Atomic Spectrometry | ROYAL SOC CHEMISTRY | Published : 2020
DOI: 10.1039/c9ja00405j
Abstract
This work introduces a new method for immuno-mass spectrometry imaging via quadrupole-based laser ablation-inductively coupled plasma-mass spectrometry instruments that is matched to the abundance of elements in biological tissues. Manipulation of ion-optics and quadrupole mass filter parameters provided increased transmission of low level high-mass elements, which are typically used as labels for antibodies, at the expense of highly abundant endogenous low-mass elements. Transmission of mid-mass elements such as transition metals was only slightly affected. The implications for mass resolution and background signals are critically discussed and signal to noise ratios and imaging capabilitie..
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Grants
Awarded by Australian Research Council
Funding Acknowledgements
D. C. was funded by the Deutsche Forschungsgemeinschaft (DFG. German Research Foundation) - 417283954. T. E. L. was supported by an Australian Government Research Training Program Stipend. P. A. D and P. A. A were recipients of an Australian Research Council Discovery Project (DP170100036). We thank Lisa Bray for the preparation of mouse tissues. The images were analysed and created using imaging software by Robin Schmid (University of Muenster). The Florey Institute of Neuroscience and Mental Health acknowledges the strong support from the Victorian Government and in particular the funding from the Operational Infrastructure Support Grant.